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Who first disproved the belief that living things arose from non living matter?

A
  

A.I. Oparin

B
  

Joseph Hooker

C
  

Robert Hooke

D
  

Louis Pasteur

Correct Answer: D

Louis Pasteur

Louis Pasteur, a brilliant French scientist, was the first person to experimentally and conclusively disprove the theory of spontaneous generation — the long-held belief that living organisms could arise spontaneously from non-living matter. For centuries, people genuinely believed that maggots grew from rotting meat, frogs emerged from mud, and bacteria appeared from thin air. Pasteur challenged this flawed belief with his famous swan-neck flask experiment conducted in 1859, which became one of the greatest experiments in the entire history of biology. He proved beyond any doubt that life only comes from pre-existing life — a principle known as biogenesis. Every student must remember that Pasteur did not just theorise — he demonstrated it practically and permanently settled this debate for the scientific world.

  • Swan-Neck Flask Experiment: Pasteur used specially designed flasks with long curved necks that allowed air but blocked dust and microorganisms from entering
  • Observation: Broth inside swan-neck flasks remained clear and sterile for long periods, proving microorganisms came from outside air, not spontaneously
  • Breaking the Flask: When the curved neck was broken, broth quickly became cloudy — confirming that microorganisms from air caused growth, not spontaneous generation
  • Biogenesis Principle: Pasteur firmly established that every living organism originates only from another pre-existing living organism
  • Other Options: Oparin proposed chemical origin of life theory, Joseph Hooker assisted Darwin, and Robert Hooke discovered the cell — none disproved spontaneous generation

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